WebFeb 12, 2024 · RIP analysis showed that Smad3, but not Smad2/4, directly bound Crnde (Fig. 4B). Most interestingly, there was no change in the effect of Crnde on Smad3 protein and phosphorylation in TGFβ-induced myofibroblast differentiation (Fig. 4C,D). It is known that Smad3 binds to the promoter region of Acta2 to regulate the expression of α-SMA … WebAug 14, 2024 · Smad3 directly induces Smad7 production, while Smad7 contributes to the degradation of Smad3. 14 In this study, renal IRI significantly induced the upregulation of TGF-β1 at both mRNA and protein levels during the AKI-to-CKD continuum. The increase of TGF-β1 resulted in the upregulation of p-Smad2, p-Smad3, and Smad4, as well as the ...
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WebSep 27, 2024 · The antibodies used for ChIP-qPCR were anti-SMAD3 (CST and C67H9), anti-SMAD4 (CST and D3R4N), and anti-USP7(Bethyl and A300-033A). All ChIP-qPCR primers are listed in Supplementary Table S1. WebMonoclonal Antibody for studying CD3epsilon. Cited in 9 publications. Validated for Western Blotting, Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, CD3ε (CD3-12) Rat Monoclonal Antibody (CST #4443) is ready to ship. chromestatus.com
Smad3 Antibody Cell Signaling Technology
WebSep 15, 2024 · Sequencings and proteomics revealed that YAP bound to Smad3 and promoted the proliferation of hBdSMC via MAPK/ERK signaling pathway in stiff ECM. Moreover, CUT and TAG sequencing and dual-luciferase assays demonstrated that Smad3 inhibited the transcription of JUN. The YAP inhibitor CA3 was used in a partial bladder … WebSep 10, 2016 · Decreased level of AZIN1 activated TGF-β1 while down-regulation of JNK1 resulted in activation of ERK and p38 kinase leading to Smad3 activation and ultimately cardiac fibrosis. Importantly, systemic neutralization of miR-433 or adeno-associated virus 9 (AAV9)-mediated cardiac transfer of a miR-433 sponge attenuated cardiac fibrosis and ... WebMar 12, 2024 · A bleomycin-induced pulmonary fibrosis animal model used SD rats. Morphological changes were analyzed by hematoxylin-eosin staining. RT-qPCR and western blot were used for the detection of the expression of TGF-β1, collagen I, collagen III, E-cadherin, fibronectin and the ratios of p-Smad2/Smad2, p-Smad3/Smad3. chrome status bar