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Human myotubes eps mtor

Web31 aug. 2024 · Myoblasts obtained from human muscle biopsies ( n = 5) were differentiated into multinucleated myotubes and exposed to 8 h of EPS consisting of 2 ms pulses at … Web25 jun. 2011 · Human myotubes were treated with leucine, insulin or co-treated with leucine and insulin for 30 min, 3 h or 24 h. Activation of mTOR signalling kinases were …

Electrical pulse stimulation of cultured human skeletal muscle cells …

Web5 nov. 2024 · Electrical pulse stimulation (EPS) has been suggested to be a useful method to investigate the mechanisms underlying the adaptations of human skeletal muscle to … WebMethods: Electrical pulse stimulation (EPS) was applied to adherent human myotubes. Cellular contents of ATP, phosphocreatine (PCr) and lactate were determined. Glucose … the swarbriggs https://amgassociates.net

Electrical pulse stimulation: an in vitro exercise model for the ...

Web15 apr. 2024 · Myotubes grown in 24-well plates (for palmitate oxidation measurement) or 6-well plates (for the other experiments) were transfected with 0.6 µg/well (24-well plates) or 2 µg/well (6-well plates) of pCMV3-ATF4 (Sino Biological Inc., Wayne, PA) or 50 mM siRNA (Santa Cruz Biotechnology Inc., Dallas, TX), using Lipofectamine 2000 (Invitrogen, … Web28 jun. 2024 · In vitro models of muscle aging are useful for understanding mechanisms of age-related muscle loss and aiding the development of targeted therapies. To investigate mechanisms of age-related muscle loss in vitro utilizing ex vivo human serum, fasted blood samples were obtained from four old (72 ± 1 yr) and four young (26 ± 3 yr) men. Older … Web28 jul. 2024 · Human myotubes from four donors were exposed to electrical pulse stimulation (EPS) or control for 48 h after which gene expression was profiled. Contributor(s) Mengeste AM, Nikolic N, Fernandez AD, Feng YZ, Nyman TA, Kersten S, Haugen F, Kase ET, Aas V, Rustan AC, Thoresen GH Citation(s) 35874526 Submission date Apr 22, … the sward wrath kung fu

Endoplasmic reticulum stress downregulates PGC-1α in skeletal …

Category:Comparative profiling of skeletal muscle models reveals …

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Human myotubes eps mtor

Differentiated C2C12 myotubes were electrically stimulated for 90 …

Web23 sep. 2024 · At this point, myotubes were nutrient deprived in Earl's Balanced Salt Solution (EBSS, ThermoFisher Scientific) for ∼14 h, with a subset of myotubes maintained in DMEM (2%HS, 1%PS) to serve as a ‘baseline’ condition. Web5 nov. 2024 · Human muscle biopsies of the vastus lateralis muscle were obtained from young, healthy, and sedentary men ( n = 3) as previously described ( Gnimassou et al., …

Human myotubes eps mtor

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Web15 apr. 2024 · Methods: The human skeletal muscle cell line LHCN-M2 and mice exposed to different inducers of ER stress were used. Results: Palmitate- or tunicamycin-induced ER stress resulted in PGC-1α downregulation and enhanced expression of activating transcription factor 4 (ATF4) in human myotubes and mouse skeletal muscle. Web1 aug. 2024 · Electrical Pulse Stimulation (EPS) of muscle cells has previously been used as an in vitro exercise model. The present study aims to establish an EPS protocol …

Web25 jun. 2011 · The actions of exogenous leucine on mTOR signalling and amino acid transporters in human myotubes mTOR signalling is transiently activated by leucine within human myotubes independently of insulin stimulation. While this occurred in the absence of changes in gene expression of amino acid transporters, protein expression of hVps34 … Webtitle = "Insight Into the Metabolic Adaptations of Electrically Pulse-Stimulated Human Myotubes Using Global Analysis of the Transcriptome and Proteome", abstract = "Electrical pulse stimulation (EPS) has proven to be a useful tool to interrogate cell-specific responses to muscle contraction.

Web19 jun. 2015 · To confirm that the EPS-induced contraction of C2C12 myotubes is an exercise-like condition, we examined AMPK phosphorylation and secretion of the myokines IL-6, MCP-1 and KC, the functional... Web1 jan. 2024 · In EPS protocol 1, the myotubes were differentiated for 6 days, washed three times with PBS, and the media were changed to serum-free differentiation media before …

WebThe present study revealed that EPS-evoked muscle contractile stimulation in 3D-EM tends to increase the concentration of lactate in the culture medium, which is consistent with previous studies...

Webskeletal muscle cells of human and animal origin. Motor neuron activation of muscle fibres can be replaced by applying EPS on differentiated skeletal muscle cells (myotubes) in culture [1,2]. Here we describe two protocols for EPS … sentences with demolishedWebmyotubes (CON myotubes) was determined at the same time points. To assess whether an increased rate of proteinsynthesisisessentialforEPS-inducedhypertrophy, myotubes … the swarm 1978 trailerWeb6 feb. 2013 · motor nerve activation is replaced by electric pulses resulting in Ca2 transients that induce de novo sarcomere assembly and contractile activities in the C2C12 myotubes (9). A recent study showed that EPS can also be applied to human myotubes to activate the sarcomere formation and contractile activity (16). the swarm 1978 ok ruWeb8 dec. 2024 · Applying EPS to differentiated myotubes mimics motor neuron activation in muscle fibers and induces myotube contraction in vitro 13. EPS also has the metabolic properties of in vivo... the swarm 1978 plotWebAdherent human myotubes were either innervated with rat spinal cord segments or exposed to EPS. The expression pattern of MyHCs was assessed by quantitative polymerase … sentences with denizenWeb20 jan. 2024 · In contrast, human myotubes differentiated on the feeder cells exhibited round cylinder-like myotubular morphology embedded within surrounding feeders and displayed obvious contractile activity... sentences with denn in germanWeb20 feb. 2024 · The mammalian target of rapamycin (mTOR) complex exerts a pivotal role in protein anabolism and cell growth. Despite its importance, few studies adequately … sentences with derive