Web31 aug. 2024 · Myoblasts obtained from human muscle biopsies ( n = 5) were differentiated into multinucleated myotubes and exposed to 8 h of EPS consisting of 2 ms pulses at … Web25 jun. 2011 · Human myotubes were treated with leucine, insulin or co-treated with leucine and insulin for 30 min, 3 h or 24 h. Activation of mTOR signalling kinases were …
Electrical pulse stimulation of cultured human skeletal muscle cells …
Web5 nov. 2024 · Electrical pulse stimulation (EPS) has been suggested to be a useful method to investigate the mechanisms underlying the adaptations of human skeletal muscle to … WebMethods: Electrical pulse stimulation (EPS) was applied to adherent human myotubes. Cellular contents of ATP, phosphocreatine (PCr) and lactate were determined. Glucose … the swarbriggs
Electrical pulse stimulation: an in vitro exercise model for the ...
Web15 apr. 2024 · Myotubes grown in 24-well plates (for palmitate oxidation measurement) or 6-well plates (for the other experiments) were transfected with 0.6 µg/well (24-well plates) or 2 µg/well (6-well plates) of pCMV3-ATF4 (Sino Biological Inc., Wayne, PA) or 50 mM siRNA (Santa Cruz Biotechnology Inc., Dallas, TX), using Lipofectamine 2000 (Invitrogen, … Web28 jun. 2024 · In vitro models of muscle aging are useful for understanding mechanisms of age-related muscle loss and aiding the development of targeted therapies. To investigate mechanisms of age-related muscle loss in vitro utilizing ex vivo human serum, fasted blood samples were obtained from four old (72 ± 1 yr) and four young (26 ± 3 yr) men. Older … Web28 jul. 2024 · Human myotubes from four donors were exposed to electrical pulse stimulation (EPS) or control for 48 h after which gene expression was profiled. Contributor(s) Mengeste AM, Nikolic N, Fernandez AD, Feng YZ, Nyman TA, Kersten S, Haugen F, Kase ET, Aas V, Rustan AC, Thoresen GH Citation(s) 35874526 Submission date Apr 22, … the sward wrath kung fu