Phospho sting molecular weight
WebMay 14, 2024 · The cyclic GMP-AMP synthase (cGAS)-stimulator of interferon genes (STING) pathway plays a pivotal role in several cellular processes including pathogen recognition and inflammatory responses. We describe a protocol to activate the cGAS-STING pathway in murine cells using nucleic acids transfection. WebMolecular Weight 33, 35 Cell Signaling Technology 3 Trask Lane Danvers, Massachusetts 01923 United States Phone: 877-616-CELL Phone: 978-867-2388 Fax: 866-432-6112 Fax: 978-867-2488 Company Profile Website: http://www.cellsignal.com/ Website: Technical Support Citations (201)
Phospho sting molecular weight
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WebWhen using primary antibodies produced in mouse to detect proteins with a molecular weight in the range of 50 kDa, we recommend using Rabbit Anti-Mouse IgG (Light Chain Specific) (D3V2A) mAb (HRP Conjugate) as a secondary antibody to minimize … WebWestern Blot analysis of Phospho-cGAS (Ser420) in various cell lines using a Phospho-cGAS (Ser420) Polyclonal antibody ( Product # PA5-121350) at a dilution of 1:1,000. 25 µg of lysate or protein were loaded into each lane. A blocking buffer of …
WebSpecificity： This antibody detects endogenous levels of STING (Phospho-Ser366) at Human, Mouse,Rat Formulation： Liquid in PBS containing 50% glycerol, and 0.111% sodium azide. Source： Polyclonal, Rabbit,IgG Dilution： WB 1:500-2000 ... Molecular Weight： ... WebSynthetic peptide corresponding to Human STAT3 (phospho S727). The antiserum was produced against synthesized phosphopeptide derived from human STAT3 around the phosphorylation site of serine 727 (P-M-SP-P …
WebHTRF Phospho and total STING enable accurate quantification of agonist-induced STING regulation. 400,000 THP1-R232 cells (from Invivogen) were plated under 25µl in 96-well plates in complete culture medium. The cells were stimulated with increasing concentrations of 2’3’cGAMP (5µL for 4 hours). WebOct 19, 2024 · The initiation of STING’s downstream transcription cascade is controlled by a multi-step process: it begins with the recruitment of TBK1, continues with the phosphorylation of STING by TBK1 and results in the engagement of interferon regulatory factor 3 (IRF3) by phosphorylated STING17,20,26,27.
WebPhospho-eNOS (Ser1177) Antibody (MA5-14957) in WB Western blot was performed using Anti-Phospho-eNOS (Ser1177) Monoclonal Antibody (H.83.2) (Product # MA5-14957) and a 160 kDa band corresponding to Phospho-eNOS (Ser1177) was observed to be upregulated upon treatment with VEGF (10 ng/mL for 60 min) and H2O2 (300 µM for 30 min).
WebKeep in mind that each phosphorylation adds 80Da to the protein molecular weight (Walsh 2006). 3 Probing for the total protein Using an antibody that detects the total level of your … early 20th century wealthy wilderness estatesWebFull length recombinant STING (TMEM173) protein produced in 293T cells. Formulation This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium … early25WebInterestingly, we found that STING interacts with both TBK1 and IRF3, and that mutations in STING that selectively disrupt its binding to IRF3 abrogate IRF3 phosphorylation without impairing TBK1 activation. These results suggest that STING functions as a scaffold to specify and promote IRF3 phosphorylation by TBK1. early 20th century women writersWebProduct Details Product Specific Information This antibody detects endogenous protein at a molecular weight of 47 and 55 kDa. Purity is >95% by SDS-PAGE. Target Information IFR3 (interferon regulatory factor 3) is a … early2bed discount codeWeb55. A method of treating, inhibiting, reducing, ameliorating, and/or preventing a cancer and or metastasis in a subject comprising administering to a subject an adoptive immunothe css table row hoverhttp://pruebas.ibt.unam.mx/publicaciones/2003/ css table show bordersWebNov 20, 2024 · Phosphorus is a mineral the body needs to build strong bones and teeth. It is also important for nerve signaling and muscle contraction. This test is ordered to see how … css table sizing